Product- Diagnostic Kits / FastSens® PCR Kit / FastSens® HSV2 PCR Kit
FastSens® HSV2 PCR Kit
FastSens® HSV 2 PCR Kit is a qualitative in vitro diagnostic product for the detection of Herpes simplex virus type2 DNA in various thermal cyclers.
+ Priciples
1) Specification
The polymerase chain reaction (PCR) DNA amplification technique is highly sensitive and Specific.
Which provides freedom in primer design and PCR optimization and maximizes PCR specificity and sensitivity by fundamentally blocking non-specific priming.
FastSens PCR Kit is a conventional PCR assay that permits the simultaneous amplification of target DNA and Internal Control (IC). The 2X Master Mix contains dNTP and enzyme for the specific amplification of the pathogens genome and 5X primer mixture for initiation of PCR.
In PCR, efficiency can be reduced by inhibitors that may be present in the clinical specimen.
The Internal Control has been added to the FastSens PCR Kit to identify processed specimens containing substances that may interfere with PCR amplification.
The Internal Control is a DNA plasmid. These features were selected to ensure equivalent amplification of the Internal Control and target DNA. The Internal Control is introduced into each amplification reaction and is co-amplified with target DNA from the clinical specimen.
In addition, the 8-methoxypsoralen (8-MOP) system is used to extinguish the template activity of contaminated DNAs. 8-MOP is known to intercalate into double-stranded nucleic acids and form a covalent interstrand crosslink after photo activation with incident light of wavelength of 320-400 nm. Please discard the PCR tube after UV irradiation (365 nm) for 20 min amplified PCR products to prevent carry-over contamination.
2) Scan Target
- Herpes simplex virus type2 DNA
3) Specimens
- Urine
- Urethal swab
- Vaginal swab
- Cervical swab
- DNA extracted from Urine and Swab specimens or DNA extracted from infected cell

+ Application to Clinical Samples


+ Contents
Components | Description | Volume |
2X HSV 2 Master Mix | – Taq-HOT DNA Polymerase – 10X Taq-HOT Buffer – 2mM each dNTPs |
500 uL |
5X Primer Mixture | – Primers for HSV Type 2 Set – Primers for Internal Control Set – Internal Control DNA |
200 uL |
HSV 2 PC | HSV Type 2 Positive Control | 50 uL |
DNA Size Marker | DNA Size Marker | 100 uL |
8-Mop Solution | To Prevent Carry-Over Contamination | 100 uL |
User Manual | 1 ea |
+ Storage
Store at – 20 ℃