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FastSens® HPV16/18 Duplex PCR Kit 2017-03-27T14:30:05+00:00

Product- Diagnostic Kits / FastSens® PCR Kit / FastSens® HPV16/18 Duplex PCR Kit

FastSens® HPV16/18 Duplex PCR Kit

FastSens® HPV 16/18 Duplex PCR Kit is a qualitative in vitro diagnostic product for the detection of Human papilloma virus type 18 and type 16 in various thermal cyclers.

+ Priciples

1) Specification

The polymerase chain reaction (PCR) DNA amplification technique is highly sensitive and Specific.
Which provides freedom in primer design and PCR optimization and maximizes PCR specificity and sensitivity by fundamentally blocking non-specific priming.
FastSens PCR Kit is a conventional PCR assay that permits the simultaneous amplification of target DNA and Internal Control (IC). The 2X Master Mix contains dNTP and enzyme for the specific amplification of the pathogens genome and 5X primer mixture for initiation of PCR.
In PCR, efficiency can be reduced by inhibitors that may be present in the clinical specimen.
The Internal Control has been added to the FastSens PCR Kit to identify processed specimens containing substances that may interfere with PCR amplification.
The Internal Control is a DNA plasmid. These features were selected to ensure equivalent amplification of the Internal Control and target DNA. The Internal Control is introduced into each amplification reaction and is co-amplified with target DNA from the clinical specimen.
In addition, the 8-methoxypsoralen (8-MOP) system is used to extinguish the template activity of contaminated DNAs. 8-MOP is known to intercalate into double-stranded nucleic acids and form a covalent interstrand crosslink after photo activation with incident light of wavelength of 320-400 nm. Please discard the PCR tube after UV irradiation (365 nm) for 20 min amplified PCR products to prevent carry-over contamination.

2) Scan Target

  • Human papilloma virus type 18 and type 16

3) Specimens

  • Urine
  • Urethal swab
  • Vaginal swab
  • Cervical swab
  • DNA extracted from Urine and Swab specimens or DNA extracted from infected cell

+ Application to Clinical Samples

+ Contents

Components Description Volume
2X HPV 16/18 Master Mix – Taq-HOT DNA Polymerase
– 10X Taq-HOT Buffer
– 2mM each dNTPs
500 uL
5X Primer Mixture – Primers for HPV Type 16 and Type 18 Set
– Primers for Internal Control Set
– Internal Control DNA
200 uL
HPV Type 16 PC HPV Type 16 Positive Control 50 uL
HPV Type 18 PC HPV Type 18 Positive Control 50 uL
DNA Size Marker DNA Size Marker 100 uL
8-Mop Solution To Prevent Carry-Over Contamination 100 uL
User Manual   1 ea

+ Storage

Store at – 20 ℃