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MG pFLAG-CMV-TOPO Vector 2017-08-21T15:14:00+00:00

Product- Molecular Reagents / Kits / MG pFLAG-CMV-TOPO Vector

MG pFLAG-CMV-TOPO Vector

+ Description

MG pFlag-CMV-TOPO Vector is suitable for cloning Taq polymerase-amplified PCR products and its protein expression in mammalian cells. It performs ligation of PCR products at room temperature in 5 min. The vector provided in this kit contains topoisomerase covalently coupled to its ends, which allows efficient ligation of DNA fragments with A-tail overhangs to the vector without using a regular ligase. The vector contains CMV promoter sequence for efficient expression of cloned gene. It also contains Flag peptide sequence as the N-terminal fusion of cloned gene so immuno-staining can be performed to verify protein expression.