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MG 2X qPCR MasterMix (SYBR Green) with ROX 2017-08-21T15:02:20+00:00

Product- Molecular Reagents / RT-PCR/Real Time PCR / MG 2X qPCR MasterMix (SYBR Green) with ROX

MG 2X qPCR MasterMix (SYBR Green) with ROX

+ Description

MG 2X qPCR Mix (SYBR Green) with ROX is formulated to achieve optimal results for a wide array of real-time PCR applications, with the convenience of SYBR Green type detection chemistry. 2x Premixed solution contains an optimized concentration of SYBR Green type dye for sensitive and accurate detection, a hot-start MG HOT DNA polymerase, and optimized reagents for qPCR. This solution provides sensitivity for HRM (High Resolution Melting). qPCR Mix (SYBR Green) kit is compatible with all real-time qPCR systems. ROX dye can be used when the real-time machine needs reference dye calibration.

Red : SYBR Green for HOT Start

Green : qPCR Mix (SYBR Green) (2X)

Sample : cDNA template synthesized from HEK293 RNA and 5pmol primer were used for the test.

PCR Condition : 95℃ 5 min / 40 cycles (95℃ 30 sec, 55℃ 30 sec, 72℃ 30 sec) / melting curve step